Dec 13, 2013 · Use of Different Parameters and Equations for Calculation of IC 50 Values in Efflux Assays: Potential Sources of Variability in IC 50 ... of the concentration vs ... 6 GraphPad Curve Fitting Guide
IC50 is the minimum inhibition concentration for inhibiting 50% of the pathogen and CC 50 is the Cytotoxic concentration of the compound to cause death to 50% of viable cells in the host. These are measured through dilution assays of the selected compound and they are added to virus infected host cells.
Calculation of EC 50. Biological responses to ligand concentrations typically following a sigmoidal function. The inflection point at which the increase in response with increasing ligand concentration begins to slow is the EC 50, which can be mathematically determined by derivation of the best-fit line.
What is the different between IC50, GI50, and ED50? Can anybody differentiate these? Its looks very similar but with different way of presentation. Cancer Therapy. Pharmaceutical Development.